dbNSFP Data Format and Usage

• We provide full dbNSFP data download in a zip file including variant tables by chromosome, gene table and search_dbNSFP annotation program.

  • dbNSFP 5.x maintains the same file formats as used in 4.x to be compatible with the existing software systems using dbNSFP data.

• We recommend using the search_dbNSFP annotation program to access the full annotation functions provided by dbNSFP and for its performance benefit when annotating a large number of variants.

• Additionally, we provide the dbNSFP variants table in BGZF format, which can be used directly with Ensembl VEP and SnpSift.

Academic Use

• dbNSFP academic branch is free for academic and non-commercial use under the CC BY-NC-ND 4.0 license.

• Academic users can download full dbNSFP data for free: please register with an academic institutional email and request download links.

• Academic users can also search and explore full dbNSFP variant and gene data in dbNSFP Web Service without the need to download.

• Some dbNSFP data (may not be up-to-date though) can also be accessed through our partners at Ensembl VEP web version, OpenCRAVAT, ANNOVAR, KGGSeq, VarSome, UCSC Genome Browser's Variant Annotation Integrator, SnpSift and HGMD.

Commercial Use

• A license is needed for commercial use of dbNSFP (c-branch). This model ensures the sustainable, long-term development of dbNSFP as a core data infrastructure for the clinical genomics and genetics research community.

  • We provide a simple flat-rate annual fee license model for application.

  • Commercial users will receive the latest dbNSFP data during their effective license period upon each release.

• Commercial users can also evaluate dbNSFP commercial branch data with free single variant query at dbNSFP Web Service.

Personal Use

• Personal users are recommended to access dbNSFP data via dbNSFP Web Service without the need of registration.